a3ar agonist cl ib meca  (Tocris)

Journal: Scientific Reports

Article Title: An adenosine A3 receptor agonist inhibits DSS-induced colitis in mice through modulation of the NF-κB signaling pathway

doi: 10.1038/srep09047

Figure Lengend Snippet: (A) IF was performed using DAPI to counterstain the nuclei and a specific antibody to label A3AR proteins. The strong green fluorescence signal indicating A3AR was observed mainly in the colonic crypt epithelial cells. The expression of A3AR was reduced in DSS colitis mice. Treatment with 2-Cl-IB-MECA showed no obvious effect on A3AR expression. A3AR mRNA (B) and protein (C) expression in colonic epithelial cells were measured by qRT-PCR and western blot, respectively. Compared with the normal group, the mRNA and protein expression levels of A3AR significantly decreased after DSS induction of colitis (* P < 0.01) and showed no significant alterations among the other three groups including DSS, DMSO, and 2-Cl-IB-MECA groups ( P > 0.05). Data are shown as mean ± SD. The blots in panel C were performed under the same experimental condition except for blotting with the different antibodies, respectively. These blots were shown as cropped images.

Article Snippet: The selective A3AR agonist 2-Cl-IB-MECA (2-Cl-IB-MECA group) (1.0 mg/kg/day in 0.2 mL) (Tocris Bioscience, United Kingdom) or 37.5% dimethyl sulfoxide (DMSO) (vehicle group) was administered to the mice intragastrically by a feeding needle once a day, starting at day 1 from colitis induction.

Techniques: Fluorescence, Expressing, Quantitative RT-PCR, Western Blot

Journal: Scientific Reports

Article Title: An adenosine A3 receptor agonist inhibits DSS-induced colitis in mice through modulation of the NF-κB signaling pathway

doi: 10.1038/srep09047

Figure Lengend Snippet: Primers used for quantitative reverse transcription-polymerase chain reaction in this study

Article Snippet: The selective A3AR agonist 2-Cl-IB-MECA (2-Cl-IB-MECA group) (1.0 mg/kg/day in 0.2 mL) (Tocris Bioscience, United Kingdom) or 37.5% dimethyl sulfoxide (DMSO) (vehicle group) was administered to the mice intragastrically by a feeding needle once a day, starting at day 1 from colitis induction.

Techniques: Reverse Transcription Polymerase Chain Reaction